9242 polyclonal rabbit anti mouse (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
9242 polyclonal rabbit anti mouse
9242 Polyclonal Rabbit Anti Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/9242+polyclonal+rabbit+anti+mouse/IkappaBalpha+Antibody/pmc13015696-66-76-82
Average 96 stars, based on 1459 article reviews
9242 Polyclonal Rabbit Anti Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/9242+polyclonal+rabbit+anti+mouse/IkappaBalpha+Antibody/pmc13015696-66-76-82
Average 96 stars, based on 1459 article reviews
9242 polyclonal rabbit anti mouse - by Bioz Stars,
2026-09
96/100 stars
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Membrane:Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling Article Snippet: Cultured cells were lysed using lysis buffer [50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40, 1 mM PMSF, and protease inhibitor cocktail] and quantified using a BCA Protein Assay kit (23227, Pierce, Rockford, IL, USA) to extract total protein, subsequently 40 μg protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). .. Polyvinylidene fluoride membrane membranes were blocked with 5% skim milk, and the following primary antibodies were used: actin (A3854, monoclonal mouse anti-mouse, Sigma-Aldrich) 1:30,000 dilution; phospho-p38 (#9211, polyclonal rabbit anti-mouse) 1:1000 dilution, p38 (#9212, polyclonal rabbit anti-mouse) 1:1000 dilution, phospho-JNK (#9255, monoclonal mouse anti-mouse) 1:1000 dilution, JNK (#9252, polyclonal rabbit anti-mouse) 1:1000 dilution, phospho-AKT (#9271, polyclonal rabbit anti-mouse) 1:1000 dilution, AKT (#9272, polyclonal rabbit anti-mouse) 1:1000 dilution, phospho-SMAD1/5/8 (#13820, monoclonal rabbit anti-mouse) 1:1000 dilution, and IκB (# Article Title: Molecular role of DRG1 in coordinating osteoclast and osteoblast differentiation during bone remodeling. Article Snippet: Cultured cells were lysed using lysis buffer [50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40, 1 mM PMSF, and protease inhibitor cocktail] and quantified using a BCA Protein Assay kit (23227, Pierce, Rockford, IL, USA) to extract total protein, Jo ur na l P re -p r of subsequently 40 μg protein was separated by 10 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). .. Polyvinylidene fluoride membrane membranes were blocked with 5 % skim milk, and the following primary antibodies were used: actin (A3854, monoclonal mouse anti-mouse, Sigma-Aldrich) 1:30,000 dilution; phospho-p38 (#9211, polyclonal rabbit anti-mouse) 1:1,000 dilution , p38 (#9212, polyclonal rabbit anti-mouse) 1:1,000 dilution, phospho-JNK (#9255, monoclonal mouse anti-mouse) 1:1,000 dilution, JNK (#9252, polyclonal rabbit anti-mouse) 1:1,000 dilution, phospho-AKT (#9271, polyclonal rabbit anti-mouse) 1:1,000 dilution, AKT (#9272, polyclonal rabbit anti-mouse) 1:1,000 dilution, phospho-SMAD1/5/8 (#13820, monoclonal rabbit anti-mouse) 1:1,000 dilution, and IκB (# |